Barley oxalate oxidase immobilized on zirconia-coated alkylamine glass using glutaraldehyde.
Indian J Biochem Biophys
;
1993 Feb; 30(1): 54-7
Artículo
en Inglés
| IMSEAR
| ID: sea-26239
ABSTRACT
A method for immobilizing barley oxalate oxidase to zirconia coated alkylamine glass through the process of glutaraldehyde coupling has been described. The immobilized enzyme retained 97.2% of the specific activity, with a conjugation yield of 6.63 mg/g support and showed an increase in optimum pH. The Km value of immobilized enzyme was unaltered but Vmax was decreased compared to free enzyme. The conjugated enzyme was stable at 4 degrees C for 2 years. A number of inorganic ions and metabolic substances did not denature the immobilized enzyme. The clinical importance of this work is demonstrated.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Oxidorreductasas
/
Plantas
/
Hordeum
/
Circonio
/
Estabilidad de Enzimas
/
Glutaral
/
Enzimas Inmovilizadas
/
Aminas
/
Concentración de Iones de Hidrógeno
Idioma:
Inglés
Revista:
Indian J Biochem Biophys
Año:
1993
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS