Optimization of extracellular xylanase production by Sclerotinia sclerotiorum S2 using factorial design.
Indian J Biochem Biophys
;
2008 Dec; 45(6): 404-9
Artículo
en Inglés
| IMSEAR
| ID: sea-26547
ABSTRACT
The improvement of xylanase production by Sclerotinia sclerotiorum S2 using a liquid fermentation culture was investigated. The optimized process was divided into three basic steps:
(i) evaluating xylanase inducers using different agricultural residues such as wheat bran, oat bran, orange peel and barley bran at 1% final concentration, and also filter paper. Among these, wheat bran showed the maximum activity (2.5 U/ml) at 12 days post-inoculation; (ii) for optimization, we determined the optimal concentration of inducer, the effect of phosphate anion (K2HPO4/KH2PO4) and culture aeration using a rotary shaker at 100 and 180 rpm. The optimal conditions for these three factors were determined in an experimental panel using factorial data, in which a mathematical model (Minitab software) was fitted; (iii) The optimized culture medium containing a high level of wheat bran (3%) without KH2PO4-K2HPO4 and submitted to a high agitation (180 rpm/min) increased the xylanase production from 2.5 U/ml to 4 U/ml (1.6-fold).
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Fosfatos
/
Ascomicetos
/
Hordeum
/
Fibras de la Dieta
/
Estadística como Asunto
/
Reactores Biológicos
/
Medios de Cultivo
/
Endo-1,4-beta Xilanasas
/
Espacio Extracelular
/
Fermentación
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
Indian J Biochem Biophys
Año:
2008
Tipo del documento:
Artículo
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