Characterization of a variant glucose-6-phosphate dehydrogenase from the south Indian population.
Indian J Biochem Biophys
;
1994 Aug; 31(4): 361-5
Artículo
en Inglés
| IMSEAR
| ID: sea-26678
ABSTRACT
Glucose-6-phosphate dehydrogenase (G6PD) is coded by a gene on the X-chromosome. Earlier studies have shown that the South Indian population has a high incidence of this enzyme deficiency. The electrophoretic mobility, pH optimum and the Km values for G6PD from normal and variant individuals were identical. However, the specific activity of the variant enzyme was 8 times less compared to the value of the normal enzyme. Western blot analysis of partially purified G6PD from normal and variant individuals performed using equal amounts of total protein showed that the variant protein was 3 times less in concentration. Similar analysis performed using protein corresponding to equal enzyme activity units in the normal and variant samples showed that the variant enzyme was 2.25 times less efficient compared to the normal enzyme. RNA dot blot analysis using full length G6PD cDNA probe (PGDT5B, a kind gift from Prof. L Luzzatto) revealed that lymphocytes from normal and variant individuals had equal amounts of G6PD specific mRNA.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Variación Genética
/
Femenino
/
Humanos
/
Masculino
/
Glucosafosfato Deshidrogenasa
/
India
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Indian J Biochem Biophys
Año:
1994
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS