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Purification of protein from a crude mixture through SDS-PAGE transfer method.
Indian J Biochem Biophys ; 2007 Apr; 44(2): 122-5
Artículo en Inglés | IMSEAR | ID: sea-26698
ABSTRACT
SDS-polyacrylamide gel electrophoresis (SDS-PAGE) transfer method was used for purification and enrichment of the protein from crude sample. Coomassie bluc/ZnSO4 stained protein band(s) containing intact polyacrylamide gel were loaded on to another polyacrylamide gel either alone or as pooled gel bands. Two/three bands were combined together and arranged tightly over one another, sealed with stacking gel and ran in another gel, which was quite useful for enrichment and purification of a particular protein from a complex mixture. Recovery of protein by gel transfer method was found to be 70% in case of ZnSO4 staining, whereas around 30% recovery was possible, following Coomassie blue staining. The method described here for purification of protein(s) from a complex mixture, following gel transfer procedure could be useful for further characterization of the desired protein.
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Espermatozoides / Coloración y Etiquetado / Testículo / Masculino / Albúmina Sérica Bovina / Bovinos / Cabras / Electroforesis en Gel de Poliacrilamida / Animales Idioma: Inglés Revista: Indian J Biochem Biophys Año: 2007 Tipo del documento: Artículo

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Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Espermatozoides / Coloración y Etiquetado / Testículo / Masculino / Albúmina Sérica Bovina / Bovinos / Cabras / Electroforesis en Gel de Poliacrilamida / Animales Idioma: Inglés Revista: Indian J Biochem Biophys Año: 2007 Tipo del documento: Artículo