Purification of protein from a crude mixture through SDS-PAGE transfer method.
Indian J Biochem Biophys
;
2007 Apr; 44(2): 122-5
Artículo
en Inglés
| IMSEAR
| ID: sea-26698
ABSTRACT
SDS-polyacrylamide gel electrophoresis (SDS-PAGE) transfer method was used for purification and enrichment of the protein from crude sample. Coomassie bluc/ZnSO4 stained protein band(s) containing intact polyacrylamide gel were loaded on to another polyacrylamide gel either alone or as pooled gel bands. Two/three bands were combined together and arranged tightly over one another, sealed with stacking gel and ran in another gel, which was quite useful for enrichment and purification of a particular protein from a complex mixture. Recovery of protein by gel transfer method was found to be 70% in case of ZnSO4 staining, whereas around 30% recovery was possible, following Coomassie blue staining. The method described here for purification of protein(s) from a complex mixture, following gel transfer procedure could be useful for further characterization of the desired protein.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Espermatozoides
/
Coloración y Etiquetado
/
Testículo
/
Masculino
/
Albúmina Sérica Bovina
/
Bovinos
/
Cabras
/
Electroforesis en Gel de Poliacrilamida
/
Animales
Idioma:
Inglés
Revista:
Indian J Biochem Biophys
Año:
2007
Tipo del documento:
Artículo
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