Ascorbate-dependent formation of hydroxyl radicals in the presence of iron chelates.
Indian J Biochem Biophys
;
1993 Oct; 30(5): 289-92
Artículo
en Inglés
| IMSEAR
| ID: sea-26876
ABSTRACT
The autoxidation of L-ascorbate on incubation in saline phosphate buffer (pH 7.4) is accompanied by hydroxyl radical (.OH) generation. The metal chelator EDTA showed significant inhibition of ascorbate autoxidation and ascorbate-dependent .OH release. On the other hand, Fe2+ (EDTA) greatly augmented both ascorbate autoxidation and ascorbate-dependent .OH production. The biological iron chelating compounds such as ATP, ADP, citrate and pyrophosphate suppressed both ascorbate autoxidation and ascorbate-mediated .OH production, thereby indicating that these compounds suppress the activating effect of iron. Ascorbate autoxidation and ascorbate-dependent .OH formation, stimulated by Fe2+ (EDTA) were significantly inhibited by .OH scavengers, namely mannitol, thiourea and sodium formate, as well as by catalase and to a lesser extent by bovine serum albumin, superoxide dismutase (native and heat denatured) and heat denatured catalase.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Oxidación-Reducción
/
Ácido Ascórbico
/
Superóxido Dismutasa
/
Tiourea
/
Cinética
/
Compuestos Ferrosos
/
Catalasa
/
Adenosina Difosfato
/
Adenosina Trifosfato
/
Quelantes del Hierro
Idioma:
Inglés
Revista:
Indian J Biochem Biophys
Año:
1993
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS