Your browser doesn't support javascript.
loading
Purification and characterisation of gelonin from seeds of Gelonium multiflorum.
Indian J Biochem Biophys ; 1999 Aug; 36(4): 258-65
Artículo en Inglés | IMSEAR | ID: sea-27072
ABSTRACT
Gelonin, a ribosome-inactivating protein has been isolated from the seeds of Gelonium multifluorum of Euphorbiaceae family by two methods and the results are compared. In method-I conventional aqueous extraction, cation-exchange and gel-filtration chromatography has been used. In method-II S-Sepharose fast flow gel has been used to purify the proteins from the seed extract, followed by ammonium sulfate fractionation, cation-exchange and gel-filtration chromatography. Extensive physico-chemical and immunological characterizations show that molecular weight of gelonin as determined by gel-filtration chromatography and SDS-PAGE is approximately 30 kDa. The non-proteinous material which binds to CMC-gel in association with gelonin in method-I is substantially removed when gelonin is purified by method-II. Cation exchange, G-100 chromatography, RP-HPLC and SDS-PAGE show that method-II yields 50% more purified gelonin when compared to the yield by method-I. The immunoreactivity of gelonin obtained by methods I and II vary from 22-26% and 50-66% respectively and the ribosome-inactivating property vary from 46-56% and 70-87% respectively.
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Proteínas de Plantas / Plantas / Semillas / Cromatografía en Gel / Cromatografía Líquida de Alta Presión / Reacciones Cruzadas / Electroforesis en Gel de Poliacrilamida / Proteínas Inactivadoras de Ribosomas Tipo 1 / Peso Molecular Idioma: Inglés Revista: Indian J Biochem Biophys Año: 1999 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Proteínas de Plantas / Plantas / Semillas / Cromatografía en Gel / Cromatografía Líquida de Alta Presión / Reacciones Cruzadas / Electroforesis en Gel de Poliacrilamida / Proteínas Inactivadoras de Ribosomas Tipo 1 / Peso Molecular Idioma: Inglés Revista: Indian J Biochem Biophys Año: 1999 Tipo del documento: Artículo