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Heat mediated quick Coomassie blue protein staining and destaining of SDS-PAGE gels.
Indian J Biochem Biophys ; 1998 Dec; 35(6): 385-9
Artículo en Inglés | IMSEAR | ID: sea-27113
ABSTRACT
For the detection of proteins on sodium dodecyl sulphate-polyacrylamide gel electrophoresis, Coomassie blue is used commonly on account of its simplicity and reliability. In this report we show that enhanced heat, in addition to dramatically decreasing the time required for staining and destaining, also significantly increased the detection sensitivity. For a 1.5 mm gel, the staining time was 5 min at 55, 62.5 or 70 degrees C while the destaining time was 45, 45 and 20 min respectively. For a 0.8 mm gel, the staining time could be reduced to 1 min at 65 degrees C compared to 2 min at 60 degrees C and 5 min at 55 degrees C. The destaining time required was 8, 15 and 20 min at the respective temperatures.
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Colorantes de Rosanilina / Coloración y Etiquetado / Factores de Tiempo / Sensibilidad y Especificidad / Colorantes / Electroforesis en Gel de Poliacrilamida / Calor Tipo de estudio: Estudio diagnóstico Idioma: Inglés Revista: Indian J Biochem Biophys Año: 1998 Tipo del documento: Artículo

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Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Colorantes de Rosanilina / Coloración y Etiquetado / Factores de Tiempo / Sensibilidad y Especificidad / Colorantes / Electroforesis en Gel de Poliacrilamida / Calor Tipo de estudio: Estudio diagnóstico Idioma: Inglés Revista: Indian J Biochem Biophys Año: 1998 Tipo del documento: Artículo