A cysteine protease of Dieffenbachia maculata.
Indian J Biochem Biophys
;
1998 Dec; 35(6): 358-63
Artículo
en Inglés
| IMSEAR
| ID: sea-27275
ABSTRACT
Plants of the genus Dieffenbachia, very popular as indoor ornamental plants, are known for their toxic as well as therapeutic properties. Their toxic manifestations have been partly attributed to their proteolytic activity. The work described in the present paper shows that stem leaves and petiole of Dieffenbachia maculata Schott, a commonly grown species, contain significant proteolytic activity, different parts showing different types of protease activities. Stem showed the highest enzyme activity and this protease was purified about 55 fold by solvent precipitation, gel filtration and ion exchange chromatography. The enzyme has a relative molecular mass of 61 kDa as determined by SDS-PAGE and has an optimum pH of 8.0 and optimum temperature of 50 degrees C. Effects of various substrates, inhibitors and activators indicate that the enzyme is a cysteine protease with leucylpeptidase activity.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Factores de Tiempo
/
Cisteína Endopeptidasas
/
Cromatografía en Gel
/
Cromatografía por Intercambio Iónico
/
Estructuras de las Plantas
/
Magnoliopsida
/
Electroforesis en Gel de Poliacrilamida
/
Peso Molecular
Idioma:
Inglés
Revista:
Indian J Biochem Biophys
Año:
1998
Tipo del documento:
Artículo
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