Spectral studies on the interaction of iodine and anthracene sulfonate with bacterial phospholipids from different mutants of Salmonella minnesota.
Indian J Biochem Biophys
;
1998 Oct; 35(5): 291-5
Artículo
en Inglés
| IMSEAR
| ID: sea-27423
ABSTRACT
Using spectrophotometric and spectrofluorometric techniques, the interaction of iodine and 2-anthracene sulfonate (ANS) with the phospholipids (PL) isolated from four genetically correlated Salmonella minnesota isolates viz., a smooth form (S), a deeply rough mutant (Rc) and two intermediate forms (Ra and Rb) were studied. Appearance of an isosbestic point and a new band in absorption spectra indicated charge-transfer (C-T) interaction of iodine with the PL through the formation of 11 complex. Stern-Volmer type fluorescence quenching of PL was observed with the addition of iodine to PL, while PL enhanced the fluorescence of anionic dye ANS. The values of the binding constants between iodine/PL and ANS/PL, measured by using suitable equations, showed a systematic gradation in the molecular properties of the PL in the membrane structure in smooth (S) and rough (Ra, Rb and Rc) mutants of Salmonella minnesota.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Fosfolípidos
/
Salmonella
/
Espectrometría de Fluorescencia
/
Espectrofotometría
/
Ácidos Sulfónicos
/
Yodo
/
Antracenos
/
Mutación
Idioma:
Inglés
Revista:
Indian J Biochem Biophys
Año:
1998
Tipo del documento:
Artículo
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