Synthesis and phosphorylation of macrophage membrane proteins in protein deficient rat.

ABSTRACT

Changes in the biosynthesis and phosphorylation of rat peritoneal macrophage membrane proteins induced by protein malnutrition have been studied. The results clearly indicate that the biosynthesis of high molecular weight proteins (45-200 kDa) and their phosphorylation are significantly reduced in the macrophages isolated from protein deficient (4% protein-fed) rats compared to the control group fed 20% protein diet. Lipopolysaccharide (LPS) treatment both in vivo and in vitro enhanced the synthesis and phosphorylation of these proteins in both control and protein deficient groups; however, the extent of enhancement was much less in the deficient group. These results indicate that besides the down regulation of these membrane proteins, protein malnutrition seems to make these macrophages less responsive to potent immuno stimulants like LPS.