Protective role of anion channel blocker in lipid peroxidation caused by H2O2 in microsomes of bovine pulmonary arterial smooth muscle tissue.
Indian J Biochem Biophys
;
1996 Feb; 33(1): 57-61
Artículo
en Inglés
| IMSEAR
| ID: sea-27573
ABSTRACT
The role of hydroxyl radical (OH.) in H2O2-mediated stimulation of lipid peroxidation in microsomes of bovine pulmonary arterial smooth muscle tissue and the protective effects of DIDS, the anion channel blocker have been studied. Treatment of microsomes with H2O2 (1 mM) stimulate iron release, OH. production and lipid peroxidation. Pretreatment with DFO (an iron chelator) or DMTU (a hydroxyl radical scavenger) prevents OH. production and thereby reduces lipid peroxidation without any appreciable reduction of iron release. Simultaneous treatment of either DFO or DMTU with H2O2 significantly reduces lipid peroxidation and prevents OH. production without any significant reduction of iron release. However, addition of DFO or DMTU 2 min after treatment of the microsome with H2O2 does not produce any significant reduction of lipid peroxidation, OH production and iron release. Pretreatment of microsomes with DIDS markedly reduces the stimulation of lipid peroxidation without appreciably altering the increase in OH. production and iron release caused by H2O2.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Arteria Pulmonar
/
Tiourea
/
Bovinos
/
Peroxidación de Lípido
/
Depuradores de Radicales Libres
/
Radical Hidroxilo
/
Ácido 4,4'-Diisotiocianostilbeno-2,2'-Disulfónico
/
Deferoxamina
/
Peróxido de Hidrógeno
/
Canales Iónicos
Idioma:
Inglés
Revista:
Indian J Biochem Biophys
Año:
1996
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS