Cloning, expression and purification of the DNA binding domain of RFX protein.
Indian J Biochem Biophys
;
1999 Dec; 36(6): 429-32
Artículo
en Inglés
| IMSEAR
| ID: sea-27899
ABSTRACT
The RFX DNA binding domain (DBD) is a novel highly conserved motif belonging to a large number of dimer DNA binding proteins which have diverse regulatory functions in eukaryotic organisms. To characterize this novel motif, a 78mer polypeptide corresponding to the DBD of human hRFX (hrfX1/DBD), a prototypical member of the RFX family has been cloned and overproduced in Escherichia coli. A purification procedure using cation exchange chromatography has also been developed.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Factores de Transcripción
/
Humanos
/
ADN
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Reacción en Cadena de la Polimerasa
/
Secuencia de Aminoácidos
/
Clonación Molecular
/
Proteínas de Unión al ADN
Idioma:
Inglés
Revista:
Indian J Biochem Biophys
Año:
1999
Tipo del documento:
Artículo
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