Comparison of refolding patterns of erabutoxin b and cardiotoxin 3.10.2 from snake venom.
Indian J Biochem Biophys
;
1994 Feb; 31(1): 20-3
Artículo
en Inglés
| IMSEAR
| ID: sea-27943
ABSTRACT
The refolding patterns of erabutoxin b (a neurotoxin) and cardiotoxin 3.10.2 (from Naja naja siamensis venom) have been studied by reducing both the proteins by treatment with reduced dithiothreitol followed by renaturation by treatment with oxidised dithiothreitol. Isoelectric focusing of the samples trapped at varying time intervals during renaturation of the proteins reveals formation of intermediates in the folding pathway with cardiotoxin 3.10.2. having fewer intermediates than erabutoxin b and faster rate of refolding (1 hr and 3 hr respectively).
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Conformación Proteica
/
Proteínas Cardiotóxicas de Elápidos
/
Venenos Elapídicos
/
Erabutoxinas
/
Neurotoxinas
Idioma:
Inglés
Revista:
Indian J Biochem Biophys
Año:
1994
Tipo del documento:
Artículo
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