An insight into the structure of DNA through melting studies.
Indian J Biochem Biophys
;
1990 Aug; 27(4): 202-8
Artículo
en Inglés
| IMSEAR
| ID: sea-27997
ABSTRACT
Helix-coil transition of calf thymus (CT) DNA in 0.1 M NaCl was observed under different environmental conditions for studying structural and conformational changes, if any. Pre-exposure of the DNA to different types of radiations - UV and gamma-brought different degrees of change (lowering) in the melting temperature (Tm). Interaction of drugs (daunomycin and actinomycin-D) increased the Tm inducing structural stability. There was 50% more destabilisation in case of polynucleotide-drug complex as compared to DNA-drug complex exposed to UV radiation. Thermodynamic studies on DNA in D2O (0.1 M NaCl prepared in D2O) were carried out and compared with the corresponding results in H2O. Presence of D2O increased the stability of the DNA structure. Structural and conformational aspects have been discussed in the light of the results obtained.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Termodinámica
/
ADN
/
Bovinos
/
Estructura Molecular
/
Animales
/
Conformación de Ácido Nucleico
/
Desnaturalización de Ácido Nucleico
Idioma:
Inglés
Revista:
Indian J Biochem Biophys
Año:
1990
Tipo del documento:
Artículo
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