Ficus cunia agglutinin for recognition of bacteria.
Indian J Biochem Biophys
;
2006 Apr; 43(2): 94-7
Artículo
en Inglés
| IMSEAR
| ID: sea-28055
ABSTRACT
Interaction of bacteria with lectin using anti-lectin antibody by ELISA is an established method. In the present study, we have devised a simple ELISA using a biotinylated lectin and antibiotin-HRP. Ficus cunia agglutinin (FCA), which has shown the specificity towards alpha/beta anomers of GlcNAc and other-NAc containing sugars like LacNAc and GlcNAcbeta(1-4/6)GlcNAc, was used as a model lectin for the study of interaction with immobilized microorganisms on ELISA plate. The bacterial cells of E. coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus subtilis and Staphylococcus aureus showed binding with FCA and the degree of binding was dependent on the bacterial surface antigen. This method is considered a simple technique to study the lectin-bacteria interaction.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
/
Ficus
/
Lectinas de Plantas
/
Bacterias Gramnegativas
/
Bacterias Grampositivas
Idioma:
Inglés
Revista:
Indian J Biochem Biophys
Año:
2006
Tipo del documento:
Artículo
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