Absorption and fluorescence studies on interaction between cationic dyes and Klebsiella K7 capsular polysaccharide.
Indian J Biochem Biophys
;
1992 Jun; 29(3): 291-5
Artículo
en Inglés
| IMSEAR
| ID: sea-28076
ABSTRACT
Interaction of cationic dyes, pinacyanol chloride, acridine orange and phenosafranin, with Klebsiella K7 capsular polysaccharide has been investigated by spectrophotometric and spectrofluorometric measurements. The acidic polysaccharide induce a metachromatic blue shift of the absorption band of pinacyanol chloride from 600 nm to 495 nm, indicating strong metachromasy. Stoichiometry of polyanion and dye cation (11.5) in the polymer-dye compound formed by the interaction between pinacyanol chloride dye and K7 polymer indicate that both glucuronic acid and pyruvic acid act as the potential anionic sites for interaction. Both spectrophotometric titration of pinacyanol chloride and spectrofluorometric titration of acridine orange and phenosafranin dyes by the polymer gave quite comparable equivalent weights for the polymer. Dye-polymer interaction studies indicated induction of metachromasy in the cationic dye by the anionic biopolymer, establishing its chromotropic character.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Polisacáridos Bacterianos
/
Espectrometría de Fluorescencia
/
Espectrofotometría
/
Cationes
/
Colorantes
/
Klebsiella
Idioma:
Inglés
Revista:
Indian J Biochem Biophys
Año:
1992
Tipo del documento:
Artículo
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