Reversible inhibition by pyridoxal 5'-phosphate and irreversible inactivation by urea and guanidine hydrochloride of thymidylate synthase from Lactobacillus leichmannii.
Indian J Biochem Biophys
;
1998 Aug; 35(4): 229-35
Artículo
en Inglés
| IMSEAR
| ID: sea-28159
ABSTRACT
Pyridoxal 5'-phosphate reversibly inhibited thymidylate synthase from Lactobacillus leichmannii. The inhibition was competitive with dUMP (Ki = 1 microM) and non-competitive with 5,10-CH2-THF (Ki = 0.08 microM). Treatment of native or pCMB-treated enzyme with urea (5 M) or guanidine hydrochloride (4 M) resulted in inactivation and dissociation of the homodimer (74 kDa) into monomer (37 kDa).
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Conformación Proteica
/
Fosfato de Piridoxal
/
Timidilato Sintasa
/
Urea
/
Dimerización
/
Guanidina
/
Inhibidores Enzimáticos
/
Lactobacillus
Idioma:
Inglés
Revista:
Indian J Biochem Biophys
Año:
1998
Tipo del documento:
Artículo
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