Specificity of antisera to sex steroid II--effect of unsaturation in ring-B of testosterone.
Indian J Biochem Biophys
;
1997 Aug; 34(4): 336-40
Artículo
en Inglés
| IMSEAR
| ID: sea-28270
ABSTRACT
In a testosterone coated tube enzyme immunoassay format, a structurally modified enzyme label 6-dehydrotestosterone-17-hemisuccinate-penicillinase (6-DT) shows lower crossreactivity (25%) towards 5 alpha-dihydrotestosterone (5 alpha-DHT) as compared to testosterone-17-hemisuccinate-penicillinase (45%). The antiserum, generated against testosterone-3-0-carboxymethyloxime-BSA conjugate, showed high crossreactivity with 5 alpha-DHT when evaluated with a RIA (44.8%) using tritiated testosterone tracer. The results suggest that the conformational changes in the new modified testosterone heterologous enzyme tracer (6-DT) brings about desired changes in crossreactivity of antisera against 5 alpha-DHT. The new assay format presents a novel approach to minimize interference due to 5 alpha-DHT crossreactivity in testosterone immunoassays.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Testosterona
/
Técnicas para Inmunoenzimas
/
Sueros Inmunes
/
Especificidad de Anticuerpos
Idioma:
Inglés
Revista:
Indian J Biochem Biophys
Año:
1997
Tipo del documento:
Artículo
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