Purification and characterization of a hemagglutinin isolated from the leaves of Chenopodium (Chenopodium amaranticolor).
Indian J Biochem Biophys
;
2001 Jun; 38(3): 186-92
Artículo
en Inglés
| IMSEAR
| ID: sea-28814
ABSTRACT
A hemagglutinin was isolated and purified from the leaves of Chenopodium (Chenopodium amaranticolor) using ion-exchange chromatography and affinity chromatography on fetuin-agarose matrix. It agglutinated rabbit erythrocytes. The hemagglutinin had a native molecular mass of 58 kDa, as estimated by gel filtration and showed a single band of molecular mass of 33 kDa on SDS-PAGE. It showed hemagglutination activity over the pH range 3-12 and was found to be stable up to 70 degrees C. On isoelectrofocussing, the pI of this hemagglutinin was estimated to be 5.25. However, it was found to contain seven charge variants when isoelectrofocussing was performed in presence of 6M urea.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Unión Proteica
/
Temperatura
/
Factores de Tiempo
/
Cromatografía en Gel
/
Cromatografía por Intercambio Iónico
/
Hojas de la Planta
/
Chenopodium
/
Electroforesis en Gel de Poliacrilamida
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Hemaglutininas
/
Concentración de Iones de Hidrógeno
Idioma:
Inglés
Revista:
Indian J Biochem Biophys
Año:
2001
Tipo del documento:
Artículo
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