Overexpression of Escherichia coli single stranded DNA binding protein under bacteriophage T 7 promoter.
Indian J Biochem Biophys
;
1994 Aug; 31(4): 249-53
Artículo
en Inglés
| IMSEAR
| ID: sea-28924
ABSTRACT
A recombinant vector for overproduction of the E. coli single stranded DNA binding protein (E. coli SSBP) has been constructed. An E. coli strain carrying this plasmid produces up to 150 mg pure SSBP per litre of bacterial culture in a laboratory shake flask. Electron microscopy of the single stranded DNA complexed with SSBP shows characteristic "beaded string"-like appearance. Strong clustering of protein molecules on ssDNA is indicative of a highly cooperative binding.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Regiones Promotoras Genéticas
/
Bacteriófago T7
/
Proteínas de Unión al ADN
/
Escherichia coli
/
Vectores Genéticos
Idioma:
Inglés
Revista:
Indian J Biochem Biophys
Año:
1994
Tipo del documento:
Artículo
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