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Effect of ischemia/reperfusion on intestinal brush border membrane lipid composition, fluidity and enzyme activities.
Indian J Biochem Biophys ; 1996 Feb; 33(1): 53-6
Artículo en Inglés | IMSEAR | ID: sea-29127
ABSTRACT
Oxygen-derived free radicals are known to be generated during ischemia/reperfusion injury and biomembranes are the prime target of these active species. In order to study the effect of in vivo generated free radicals on intestinal mucosal membrane, brush border membranes (BBM) were isolated from rat small intestine after subjecting to ischemia (I) and ischemia/reperfusion (I/R) injury and their lipid composition and marker enzyme activity were compared with BBM prepared from control animals. No significant alteration in the lipid composition of BBM was observed after I or I/R as compared to control. Membrane fluidity measurements showed that I/R increased the fluidity of BBM. Activity of alkaline phosphatase, one of the marker enzymes for BBM was reduced by I or I/R whereas activity of another BBM enzyme, sucrase was not altered. The decrease in alkaline phosphatase activity was more after reperfusion. In vitro fluidization of BBM using benzyl alcohol indicated that the inactivation of alkaline phosphatase was not due to change in fluidity. These results suggest that free radicals generated during I/R inactivate BBM alkaline phosphatase partially without altering the membrane lipid composition.
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Ratas / Valores de Referencia / Sacarasa / Reperfusión / Fosfatasa Alcalina / Mucosa Intestinal / Intestinos / Isquemia / Animales / Fluidez de la Membrana Idioma: Inglés Revista: Indian J Biochem Biophys Año: 1996 Tipo del documento: Artículo

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Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Ratas / Valores de Referencia / Sacarasa / Reperfusión / Fosfatasa Alcalina / Mucosa Intestinal / Intestinos / Isquemia / Animales / Fluidez de la Membrana Idioma: Inglés Revista: Indian J Biochem Biophys Año: 1996 Tipo del documento: Artículo