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Multi-dot enzyme-linked immunosorbent assay for serodiagnosis of trematodiasis.
Southeast Asian J Trop Med Public Health ; 1990 Sep; 21(3): 471-4
Artículo en Inglés | IMSEAR | ID: sea-30971
ABSTRACT
A nitrocellulose membrane strip dotted with crude antigens of trematodes, such as Paragonimus miyazakii, P. westermani, Fasciola sp., Clonorchis sinensis and Schistosoma japonicum was applied for serodiagnosis of trematodiasis. With peroxidase conjugated anti-human IgG and 4-chloro-1-naphthol as a substrate, antibodies in trematodiasis patient sera were clearly detected on the strip as bluish purple spots. Densities of the spots correlated well with values obtained by standard micro-ELISA. Cross-reactions among the trematode antigens, which is occurred more frequently than by ELISA, were observed with this method. However, the darkest spot correlated with the infecting parasite in all cases. With the high reliability and advantage that crude antigens can be used, this simple method, multi-dot ELISA, is considered to be useful for serodiagnosis of trematodiasis.
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Trematodos / Infecciones por Trematodos / Ensayo de Inmunoadsorción Enzimática / Pruebas Serológicas / Anticuerpos Antihelmínticos / Reacciones Cruzadas / Animales Tipo de estudio: Estudio diagnóstico Idioma: Inglés Revista: Southeast Asian J Trop Med Public Health Año: 1990 Tipo del documento: Artículo

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Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Trematodos / Infecciones por Trematodos / Ensayo de Inmunoadsorción Enzimática / Pruebas Serológicas / Anticuerpos Antihelmínticos / Reacciones Cruzadas / Animales Tipo de estudio: Estudio diagnóstico Idioma: Inglés Revista: Southeast Asian J Trop Med Public Health Año: 1990 Tipo del documento: Artículo