Molecular identification of Taenia solium cysticercus genotype in the histopathological specimens.
Southeast Asian J Trop Med Public Health
;
2005 ; 36 Suppl 4(): 131-4
Artículo
en Inglés
| IMSEAR
| ID: sea-31288
ABSTRACT
PCR-based molecular diagnosis was done for identification of causative agents found in paraffin-embedded specimens that were resected from two suspected neurocysticercosis patients. DNA samples were extracted from tissues or sections and cytochrome c oxidase subunit 1 gene and cytochrome b gene were amplified for the detection of taeniid DNA. Two different genes were successfully amplified in both specimens, but the sizes of amplified products seemed to depend on the quality of DNA. Based on the nucleotide sequences of the PCR-amplified genes, the causative agents from two cases were identified as T. solium Asian genotype. When infection with T. solium is not confirmed by histopathological examination, molecular diagnosis will be more useful for definitive diagnosis.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Especificidad de la Especie
/
Manejo de Especímenes
/
Teniasis
/
Femenino
/
Humanos
/
Niño
/
Reacción en Cadena de la Polimerasa
/
Neurocisticercosis
/
Taenia solium
/
Genotipo
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
Southeast Asian J Trop Med Public Health
Año:
2005
Tipo del documento:
Artículo
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