Comparative study of Dot-immunogold silver staining and Dot-ELISA for the detection of serum antibodies against Wuchereria bancrofti.
Southeast Asian J Trop Med Public Health
;
1994 Dec; 25(4): 724-7
Artículo
en Inglés
| IMSEAR
| ID: sea-31315
ABSTRACT
Dot-immunogold silver staining (Dot-IGSS) and Dot-ELISA, using the soluble antigen of Brugia malayi, were employed to detect anti-Wuchereria bancrofti antibodies in 50 cases of Wuchereria bancrofti microfilaremia. The positive rates were 100% and 90% in Dot-IGSS and Dot-ELISA respectively. The average titer in the 45 positive cases was 1184 (110-12560) for Dot-IGSS and 1150 (110-12560) for Dot-ELISA, with 30 cases showing the same titer in both tests, 13 cases showing higher titer in Dot-IGSS than in Dot-ELISA and 2 cases in the former showing lower titers than in the latter. There was a linear relationship between the titers of antibodies detected by Dot-IGSS and by Dot-ELISA (r = 0.8443). Dot-IGSS, similar to Dot-ELISA, is easy to carry out and the result is easy to read. It is seen that Dot-IGSS is highly sensitive and specific and is practicable for immunodiagnosis and surveillance of filariasis.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Wuchereria bancrofti
/
Filariasis Linfática
/
Humanos
/
Ensayo de Inmunoadsorción Enzimática
/
Anticuerpos Antihelmínticos
/
Inmunohistoquímica
/
Immunoblotting
/
Valor Predictivo de las Pruebas
/
Animales
/
Microfilarias
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Idioma:
Inglés
Revista:
Southeast Asian J Trop Med Public Health
Año:
1994
Tipo del documento:
Artículo
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