Characterization of a monoclonal antibody that neutralizes the hyaluronidase activity of Russell's viper venom.
Southeast Asian J Trop Med Public Health
;
1990 Jun; 21(2): 231-7
Artículo
en Inglés
| IMSEAR
| ID: sea-31704
ABSTRACT
Three monoclonal antibodies (WPN1, WPN2 and WPN3) raised against a partially purified fraction of Russell's viper venom (RVV) were characterized. All three monoclonal antibodies reacted with crude RVV when tested by ELISA, but only two (WPN1, WPN2) neutralized its hyaluronidase activity. WPN1 was the more potent and was effective at an antigen antibody ratio of 13. Furthermore, WPN1 was shown to recognize only the 14,000 MW component of crude RVV. This has been identified in a previous study to be hyaluronidase. This antibody was also found to recognize some components of Calloselasma rhodostoma venom which also possesses potent hyaluronidase activity. The potential therapeutic role of antibodies that neutralize the hyaluronidase component of snake venoms should be investigated further.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Humanos
/
Ensayo de Inmunoadsorción Enzimática
/
Immunoblotting
/
Venenos de Crotálidos
/
Hialuronoglucosaminidasa
/
Anticuerpos Monoclonales
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
Southeast Asian J Trop Med Public Health
Año:
1990
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS