Studies on the inhibitory activities of human serum lipoproteins for Japanese encephalitis virus.
Southeast Asian J Trop Med Public Health
;
1975 Dec; 6(4): 461-6
Artículo
en Inglés
| IMSEAR
| ID: sea-32224
ABSTRACT
Human serum lipoproteins were purified by ultracentrifuging and their concentrations adjusted as required to be within the normal male/female serum range for all assays. The activities in inhibition of hemagglutination (HAI) for Japanese encephalitis virus were--low density lipoprotein (LDL) greater than very low density lipoprotein (VLDL) greater than high density lipoprotein (HDL). Heating (56 degrees C/30 minutes) caused the LDL titer to fall and freeze-thawing (20 degrees C/room temperature) the VLDL titer to rise slightly, possibly as a result of alteration in lipoprotein structure. The additon of lipoprotein depleted serum appeared to dampen these effects and there was no nett change in titer when it was added to a lipoprotein mixture. Similarly, unfractionated normal serum showed no significant change in titer after these treatments. The lipoproteins lacked significant virus neutralizing (VN) activity and this remained so in spite of fluctuations in HAI titer after heating and freeze-thawing.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Antivirales
/
Humanos
/
Masculino
/
Pruebas de Inhibición de Hemaglutinación
/
Encefalitis Japonesa
/
Virus de la Encefalitis Japonesa (Especie)
/
Congelación
/
Calefacción
/
Lipoproteínas
/
Lipoproteínas HDL
Idioma:
Inglés
Revista:
Southeast Asian J Trop Med Public Health
Año:
1975
Tipo del documento:
Artículo
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