Partially purified antigens of Paragonimus heterotremus for serodiagnosis of human paragonimiasis.
Southeast Asian J Trop Med Public Health
;
1994 Mar; 25(1): 176-80
Artículo
en Inglés
| IMSEAR
| ID: sea-32985
ABSTRACT
The preparative crude extract of Paragonimus heterotremus was fractionated by isoelectric focusing. Fractions at pH 5 which contained a specific antigen with a relative molecular weight of 31.5 kDa were pooled and used in an indirect enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis for diagnosis of human paragonimiasis. The sensitivity and specificity of ELISA were found to be 100% and 99% respectively. The band of 31.5 kDa antigenic component was found to give consistent reaction with paragonimiasis sera. The sensitivity, specificity and predictive value (positive and negative) of immunoblot analysis for the 31.5 kDa band were all 100%.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Paragonimiasis
/
Paragonimus
/
Humanos
/
Ensayo de Inmunoadsorción Enzimática
/
Pruebas Serológicas
/
Immunoblotting
/
Fraccionamiento Celular
/
Sensibilidad y Especificidad
/
Concentración de Iones de Hidrógeno
/
Focalización Isoeléctrica
Tipo de estudio:
Ensayo Clínico Controlado
/
Estudio diagnóstico
/
Estudio pronóstico
Idioma:
Inglés
Revista:
Southeast Asian J Trop Med Public Health
Año:
1994
Tipo del documento:
Artículo
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