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Preparation of monoclonal antibody against Angiostrongylus cantonensis antigen.
Southeast Asian J Trop Med Public Health ; 1989 Mar; 20(1): 119-24
Artículo en Inglés | IMSEAR | ID: sea-33379
ABSTRACT
Monoclonal antibodies (MAb) against A cantonensis were produced through fusion of immunised spleen cells from BALB/c mice with NS-1 myeloma cells at a ratio of 101. The successful fusion rate on the 3rd day of fusion was 90.1%. Ten MAb were characterised, six of which were IgG1 and the remaining four were IgG2a, IgG2b, IgM and IgA respectively. Among 6 IgG1 MAb, four were A. cantonensis-specific, of which three reacted to adult worm antigen only and one reacted to both adult worm and juvenile worm antigens. Two other IgG1 MAb showed cross-reaction with other helminthic antigens of Toxocara canis. Ascaris suum. Paragonimus westermani, Dirofilaria immitis, Anisakis Spp, Gnatostoma Spinigerum and Clonorchis sinensis.
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Femenino / Ensayo de Inmunoadsorción Enzimática / Hibridomas / Angiostrongylus / Animales / Metastrongyloidea / Ratones / Ratones Endogámicos BALB C / Anticuerpos Monoclonales / Antígenos Helmínticos Idioma: Inglés Revista: Southeast Asian J Trop Med Public Health Año: 1989 Tipo del documento: Artículo

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Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Femenino / Ensayo de Inmunoadsorción Enzimática / Hibridomas / Angiostrongylus / Animales / Metastrongyloidea / Ratones / Ratones Endogámicos BALB C / Anticuerpos Monoclonales / Antígenos Helmínticos Idioma: Inglés Revista: Southeast Asian J Trop Med Public Health Año: 1989 Tipo del documento: Artículo