Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.
Southeast Asian J Trop Med Public Health
;
1989 Dec; 20(4): 519-22
Artículo
en Inglés
| IMSEAR
| ID: sea-34669
ABSTRACT
A simple procedure was developed for spotting blood samples directly onto nylon membrane filter, without the necessity to treat samples with pronase or proteinase K, followed by hybridizing with 32P-labelled DNA probe, pUNK1-45. This probe detected specifically P. falciparum DNA and did not cross react with DNA from man, P. knowlesi, P. chabaudi or P. cynomolgi. The probe was sensitive to detect a parasitemia of 0.001% in 20 microliters of blood.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Radioisótopos de Fósforo
/
Plasmodium falciparum
/
Humanos
/
Sondas de ADN
/
Métodos Epidemiológicos
/
Animales
/
Malaria
/
Hibridación de Ácido Nucleico
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Southeast Asian J Trop Med Public Health
Año:
1989
Tipo del documento:
Artículo
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