Prevalence of HIV-1 polymerase gene mutations in pre-treated patients in Thailand.
Southeast Asian J Trop Med Public Health
;
2002 Mar; 33(1): 80-4
Artículo
en Inglés
| IMSEAR
| ID: sea-35045
ABSTRACT
To determine the prevalence of drug resistance-conferring mutations in human immunodeficiency virus type 1 (HIV-1), 83 HIV-1 infected Thai patients who had been treated with any antiretroviral drug were studied. HIV-1 RNA was reverse transcribed and amplified by RT-PCR. The direct sequencing of HIV-1 reverse transcriptase (RT) and protease was then performed. Changes in nucleotide and amino acid sequences were determined by comparison with a pNL4-3 reference sequence. Data on mutations associated with resistance to antiretroviral drugs were obtained from literature. The mutations associated with lamivudine resistance (M184V/I) were found most often (in 45.7% of individuals). Zidovudine-resistant mutants T215Y/F (36%), M41L (28%) and K70R (25.3%) were common; but mutations linked to didanosine (L74V) and multinucleoside-resistant genotypes (Q151M) were rarely recognized (2.4% and 3.6%, respectively). The stavudine-resistant mutant (V75T) and T69 insertions were not found. All subjects who had a significant exposure to antiretroviral drugs and current virological failure in the past carried drug-resistant genotypes. Genotypic resistance to zidovudine, lamivudine, zalcitabine, indinavir and ritonavir appeared in more than one third of the samples, which suggested that the prevalence of the HIV-1 resistance-conferring genotype resisting reverse transcriptase inhibitors and/or protease inhibitors was high in treatment experienced patients.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Filogenia
/
Tailandia
/
Humanos
/
ARN Polimerasas Dirigidas por ADN
/
Infecciones por VIH
/
Proteasa del VIH
/
VIH-1
/
Inhibidores de la Proteasa del VIH
/
Inhibidores de la Transcriptasa Inversa
/
Farmacorresistencia Viral
Tipo de estudio:
Estudio de prevalencia
/
Factores de riesgo
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Southeast Asian J Trop Med Public Health
Año:
2002
Tipo del documento:
Artículo
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