Methylene blue staining method for identification of Opisthorchis viverrini egg.
Southeast Asian J Trop Med Public Health
;
2005 ; 36 Suppl 4(): 107-9
Artículo
en Inglés
| IMSEAR
| ID: sea-35287
ABSTRACT
Methylene blue staining method was used to distinguish O. viverrini eggs from Haplorchis taichui and Prosthodendrium molenkampi eggs. All eggs were obtained from dissected adult worms, fixed in 10% formalin, and stained with methylene blue prior to light microscopy observation. The distinct musk-melon-like texture of the O. viverini eggshell surface and the thread-like texture of H. taichui eggshell surface were recognized, while P. molenkampi eggs showed a smooth eggshell. We also evaluated the sensitivity and specificity of the method by training investigators to differentiate surface textures. After training, the investigators were randomly tested with 10 slides containing fluke eggs. The sensitivity and specificity were 95% and 95%, respectively.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Opistorquiasis
/
Opisthorchis
/
Óvulo
/
Especificidad de la Especie
/
Coloración y Etiquetado
/
Humanos
/
Sensibilidad y Especificidad
/
Colorantes
/
Animales
/
Azul de Metileno
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Idioma:
Inglés
Revista:
Southeast Asian J Trop Med Public Health
Año:
2005
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS