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Molecular examination of GH gene deletion in familial growth hormone deficiency.
Southeast Asian J Trop Med Public Health ; 1995 ; 26 Suppl 1(): 207-11
Artículo en Inglés | IMSEAR | ID: sea-35509
ABSTRACT
The human growth hormone gene (GH gene) from nine members of a family with familial growth hormone deficiency was examined. The patients were diagnosed as having growth hormone deficiency clinically and by response to hormonal treatment. PCR amplification was carried out using total DNA extracted from leukocytes. The flanking regions of the GH gene which are highly homologous were amplified by one pair of primers. PCR products of 1900 bp and 1919 bp were obtained. By using the combination of restriction enzymes BgII, HaeII and SmaI to digest these PCR products, the various sizes of GH gene deletion can be detected. None of the possible deletions was found in these patients and their relatives by either PCR or Southern blot analysis.
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Femenino / Humanos / Masculino / Hormona del Crecimiento / Mapeo Restrictivo / Preescolar / Southern Blotting / Reacción en Cadena de la Polimerasa / Eliminación de Gen / Trastornos del Crecimiento Idioma: Inglés Revista: Southeast Asian J Trop Med Public Health Año: 1995 Tipo del documento: Artículo

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Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Femenino / Humanos / Masculino / Hormona del Crecimiento / Mapeo Restrictivo / Preescolar / Southern Blotting / Reacción en Cadena de la Polimerasa / Eliminación de Gen / Trastornos del Crecimiento Idioma: Inglés Revista: Southeast Asian J Trop Med Public Health Año: 1995 Tipo del documento: Artículo