Dot enzyme immunosorbent assay for the serodiagnosis of typhoid fever.
Southeast Asian J Trop Med Public Health
;
1991 Dec; 22(4): 563-6
Artículo
en Inglés
| IMSEAR
| ID: sea-35620
ABSTRACT
A nitrocellulose membrane strip dotted with a specific 50 kDa outer membrane protein of Salmonella typhi was applied for the serodiagnosis of typhoid fever. Using horseradish peroxidase conjugated IgM and IgG antibodies with 4-chloronaphthol as substrate, antibodies in typhoid patients were clearly visualised as bluish purple dots while sera from patients with non-typhoid fevers gave negative results. The detection of specific IgM and IgG antibodies in typhoid patients suggest either recent or current infection. Combined with the high specificity, reliability and rapidity of the test, the dot EIA technique provides a simple and useful method for the serodiagnosis of typhoid using a single serum specimen.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Salmonella typhi
/
Fiebre Tifoidea
/
Humanos
/
Inmunoglobulina G
/
Inmunoglobulina M
/
Ensayo de Inmunoadsorción Enzimática
/
Pruebas Serológicas
/
Peroxidasa de Rábano Silvestre
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Southeast Asian J Trop Med Public Health
Año:
1991
Tipo del documento:
Artículo
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