Detection and molecular characterization of the zot gene in Vibrio cholerae and V. alginolyticus isolates.
Southeast Asian J Trop Med Public Health
;
2001 Mar; 32(1): 100-4
Artículo
en Inglés
| IMSEAR
| ID: sea-35683
ABSTRACT
A total of 11 Vibrio cholerae isolates from 1996-1998 outbreaks in Malaysia and 4 V. alginolyticus were analyzed. Isolates were characterized by polymerase chain reaction (PCR) and Southern hybridization for the presence of the gene encoding zonula occludens toxin (zot). Screening of zot gene by PCR revealed the presence of this gene in V. cholerae and V. alginolyticus. The zot gene from one V. cholerae Ogawa isolate that was cloned in a pCR 2.1 TOPO vector was sequenced. The sequences obtained were 99% homologous to the zot gene sequence from the Gene Bank.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Especificidad de la Especie
/
Vibrio
/
Vibrio cholerae
/
Humanos
/
ADN Bacteriano
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Homología de Secuencia de Ácido Nucleico
/
Toxina del Cólera
/
Southern Blotting
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Southeast Asian J Trop Med Public Health
Año:
2001
Tipo del documento:
Artículo
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