SCCmec typing and detection of VISA-related genes in methicillin-resistant Staphylococcus aureus clinical strains from Kobe University Hospital, Japan.
Southeast Asian J Trop Med Public Health
;
2006 Nov; 37(6): 1149-55
Artículo
en Inglés
| IMSEAR
| ID: sea-35752
ABSTRACT
A total of 50 clinical strains of methicillin-resistant Staphylococcus aureus (MRSA) were collected from Kobe University Hospital in 2003. Molecular typing of SCCmec was performed by multiplex polymerase chain reaction (PCR) and the presence of six genes (vraR, vraG, vraA, vraF, fruA, and fruB) associated with vancomycin (VCM) resistance was examined by simple PCR analysis. Out of 50 MRSA strains isolated 47 strains contained Type II SCCmec and the remaining contained Type IV SCCmec. Thirty seven strains contained pUB110 plasmid. VraA was present in 69% of the strains, vraF in 10%, vraG in 53%, and vraR in 16%. Noteworthy, strains without pUB110 contained vraR in relatively higher frequency (31%) compared with strains with pUB110 (11%).
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Infecciones Estafilocócicas
/
Staphylococcus aureus
/
Proteínas Bacterianas
/
Humanos
/
Datos de Secuencia Molecular
/
Pruebas de Sensibilidad Microbiana
/
Reacción en Cadena de la Polimerasa
/
Resistencia a la Meticilina
/
Técnicas de Tipificación Bacteriana
/
Cromosomas Bacterianos
Tipo de estudio:
Estudio diagnóstico
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Southeast Asian J Trop Med Public Health
Año:
2006
Tipo del documento:
Artículo
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