A rapid and simplified ELISA using whole blood samples of Schistosoma japonicum-infected rabbits.
Southeast Asian J Trop Med Public Health
;
2005 Mar; 36(2): 445-50
Artículo
en Inglés
| IMSEAR
| ID: sea-35863
ABSTRACT
A rapid and simplified ELISA using whole blood samples of Schistosoma japonicum-infected rabbits was compared with a conventional ELISA. This whole-blood ELISA has advantages. The volume of crude egg antigens, whole blood samples, and conjugates was only 0.05 ml. The incubation time was shortened to 5 minutes. Wells were washed three to five times with PBS-Tween after each procedure. Optical density values were measured in 10 minutes after transfer of 0.1 ml of substrate. Constant temperature was not necessary. The entire procedure took only 20-30 minutes.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Conejos
/
Schistosoma japonicum
/
Esquistosomiasis
/
Factores de Tiempo
/
Ensayo de Inmunoadsorción Enzimática
/
Tamizaje Masivo
/
Animales
/
Antígenos Helmínticos
Tipo de estudio:
Estudios de evaluación
/
Estudio pronóstico
/
Estudio de tamizaje
Idioma:
Inglés
Revista:
Southeast Asian J Trop Med Public Health
Año:
2005
Tipo del documento:
Artículo
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