The diagnosis of human opisthorchiasis.
Southeast Asian J Trop Med Public Health
;
2002 ; 33 Suppl 3(): 86-91
Artículo
en Inglés
| IMSEAR
| ID: sea-36047
ABSTRACT
Opisthorchiasis viverrini is a liver fluke infection causing a serious public health problem in Thailand, Lao PDR, Cambodia, and South Vietnam because it acts as a strong promoter of cholangiocarcinoma. The diagnosis of human opisthorchiasis is based on four approaches clinical manifestations, parasitological, molecular biological, and immunological methods. These methods have advantages and disadvantages. Clinical manifestations of the patients are practically indistinguishable from those of other liver diseases. The features of the O. viverrini eggs are, by light microscopy, difficult to differentiate from those of other minute intestinal flukes' eggs. Polymerase chain reaction (PCR) is very complicated, needs special and expensive apparatus, and is time-consuming; it is, however, highly sensitive and specific. Immunological testing is the method of choice the techniques are applicable to both routine laboratory work and field or epidemiological studies. Of these tests, enzyme-linked immunosorbent assay (ELISA) and immunoelectrotransfer blot assay are often used for the detection of O. viverrini-specific antigens (coproantigens) and antibodies (IgM, IgG, IgA, or IgE). Monoclonal antibodies are prepared to detect coproantigens, while the crude somatic and excretory-secretory antigens from the adult worms, metacercariae, eggs, and snail intermediate hosts are prepared in order to detect antibodies in sera. To eliminate the cross reactions between parasites, the appropriate amount, type, and efficacy of antigens or antibodies preparation should be considered. In this paper, the advantages and disadvantages of the four diagnostic methods are discussed.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Opistorquiasis
/
Recuento de Huevos de Parásitos
/
Tailandia
/
Humanos
/
Ensayo de Inmunoadsorción Enzimática
/
Pruebas de Hemaglutinación
/
Western Blotting
/
Reacción en Cadena de la Polimerasa
/
Sensibilidad y Especificidad
/
ADN de Helmintos
Tipo de estudio:
Estudio diagnóstico
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Southeast Asian J Trop Med Public Health
Año:
2002
Tipo del documento:
Artículo
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