Comparative study of anti-double stranded DNA detection by ELISA and Crithidia luciliae immunofluorescence.
Southeast Asian J Trop Med Public Health
;
2003 Sep; 34(3): 646-50
Artículo
en Inglés
| IMSEAR
| ID: sea-36122
ABSTRACT
Two commercial enzyme-linked immunosorbent assays (ELISA) and two commercial Crithidia luciliae immunofluorescence tests (CLIF) were reevaluated as to the efficiency and degree of correlation of anti-double stranded DNA (anti-dsDNA) detection in systemic lupus erythematosus (SLE). The two ELISAs exhibited an overall agreement of 95% and significantly correlated with each other (r=0.91, p<0.001). They were comparable in sensitivity (64%, 61%) and had the same specificity (95%, 95%). The sensitivity of the two CLIFs was 39% and 29% with corresponding specificities of 100% and 97%, and an overall agreement with each other of 94%. The two ELISAs had comparable specificity to the CLIFs with good agreement (84%, 79%) while they had a much greater sensitivity than the CLIFs. These findings suggest that ELISA is a useful laboratory test for anti-dsDNA detection of SLE due to its simplicity, quantitative results, sensitivity, specificity and cost, as compared to CLIFs.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Juego de Reactivos para Diagnóstico
/
Humanos
/
Ensayo de Inmunoadsorción Enzimática
/
Estudios de Casos y Controles
/
Modelos Lineales
/
Anticuerpos Antinucleares
/
Valor Predictivo de las Pruebas
/
Técnica del Anticuerpo Fluorescente
/
Sensibilidad y Especificidad
/
Lupus Eritematoso Sistémico
Tipo de estudio:
Estudio diagnóstico
/
Estudio observacional
/
Estudio pronóstico
/
Factores de riesgo
Idioma:
Inglés
Revista:
Southeast Asian J Trop Med Public Health
Año:
2003
Tipo del documento:
Artículo
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