Immuno-histological characterization of OVS1 and OVS2 monoclonal antibodies recognizing human ovarian mucinous cystadenocarcinoma.
Asian Pac J Allergy Immunol
;
1992 Dec; 10(2): 129-34
Artículo
en Inglés
| IMSEAR
| ID: sea-36645
ABSTRACT
OVS1 and OVS2 monoclonal antibodies (MAbs) were established by fusing murine myeloma cell line NS1/1-Ag4-1 with mouse spleen cells immunized with fresh human ovarian mucinous-cystadenocarcinoma tissue. The selection of the MAbs was assayed by an immuno-histological (streptavidin-biotin) staining of the specific antigen antibody reaction localized on frozen sections of the same tumor. Other paraffin sections and established cell lines were also screened by immuno-histological staining in order to characterize the specificity and sensitivity of these two MAbs. OVS1 MAb showed 96% specificity and 67% sensitivity to mucinous cystadenocarcinoma with no cross reactions to normal tissue, benign tissue, other cancers, or any established cell lines. OVS2 MAb revealed only 8% specificity but 78% sensitivity to mucinous cystadenocarcinoma, however, a cross reaction to some normal and benign tissues or other cancers was shown. The data suggested that OVS1 and OVS2 MAbs could be used in combination to detect ovarian mucinous cystadenocarcinoma.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Neoplasias Ováricas
/
Coloración y Etiquetado
/
Femenino
/
Humanos
/
Masculino
/
Células Tumorales Cultivadas
/
Técnicas para Inmunoenzimas
/
Sensibilidad y Especificidad
/
Cistadenocarcinoma
/
Animales
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Asian Pac J Allergy Immunol
Año:
1992
Tipo del documento:
Artículo
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