A novel positively-charged lipid 1,2-bis(hexadecylcycloxy)-3-trimethyl aminopropane (BisHOP) enhances the adjuvant effect of liposomes on encapsulated tetanus toxoid.
Asian Pac J Allergy Immunol
;
1991 Jun; 9(1): 21-4
Artículo
en Inglés
| IMSEAR
| ID: sea-36982
ABSTRACT
A novel positively charged lipid, 1,2-bis(hexadecylcycloxy)-3-trimethylaminopropane-HCl (BisHOP), when incorporated into the bilayers of phosphatidylcholine (PC) and distearoyl phosphatidylcholine (DSPC) dehydration-rehydration vesicles (DRV), was shown to have a powerful effect in enhancing the IgG1 response to tetanus toxoid encapsulated within the liposomes. The adjuvant effect was significantly greater when 20% BisHOP was incorporated as compared to 10% incorporation and to control PC and DSPC DRV. Plain, uncharged DSPC DRV were found to have a greater adjuvant effect than plain PC DRV on the entrapped tetanus toxoid after a single intramuscular injection. Even though antibody levels at 8 weeks post-injection were similar for 20% BisHOP PC and DSPC DRV, the rate of rise of antibody titres was more rapid for 20% BisHOP DSPC than for 20% BisHOP PC DRV. These results suggest that faster and higher titers of antibodies may be obtained by optimal manipulation of the charged and non-charged lipid components of liposomes.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Inmunoglobulina G
/
Cápsulas
/
Portadores de Fármacos
/
Toxoide Tetánico
/
Adyuvantes Inmunológicos
/
Compuestos de Amonio Cuaternario
/
Lípidos
/
Liposomas
/
Animales
/
Ratones
Idioma:
Inglés
Revista:
Asian Pac J Allergy Immunol
Año:
1991
Tipo del documento:
Artículo
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