Comparative study of respiratory syncytial virus in nasopharyngeal aspirates using conventional cell culture, shell viral centrifugation culture, immunofluorescence and biotin-avidin enzyme linked immunosorbent assays.
Asian Pac J Allergy Immunol
;
1991 Dec; 9(2): 121-4
Artículo
en Inglés
| IMSEAR
| ID: sea-37056
ABSTRACT
133 nasopharyngeal aspirates (NPA) were simultaneously tested for the presence of respiratory syncytial virus (RSV) by conventional cell culture (CCC), shell vial centrifugation culture (SVC), immunofluorescence assay (IFA) and biotin-avidin enzyme linked immunosorbent assay (B-A ELISA). These yielded positive results in 32(24%), 45(33.8%), 36(27%) and 40(30%) of specimens, respectively. Specimens positive by IFA and B-A ELISA were all also positive by SVC. The sensitivity of CCC, IFA, and B-A ELISA comparing to SVC was 71%, 80%, and 88.9%, respectively. For rapid detection of RSV, we recommend the SVC method where a cell culture laboratory is available and the B-A ELISA method where a cell culture laboratory is not available.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Infecciones por Respirovirus
/
Virus Sincitiales Respiratorios
/
Humanos
/
Ensayo de Inmunoadsorción Enzimática
/
Centrifugación
/
Nasofaringe
/
Técnica del Anticuerpo Fluorescente
/
Sensibilidad y Especificidad
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Asian Pac J Allergy Immunol
Año:
1991
Tipo del documento:
Artículo
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