Antinuclear antibody detection using streptavidin-biotin-peroxidase complex on HEp-2 cell substrate.
Asian Pac J Allergy Immunol
;
1992 Jun; 10(1): 19-24
Artículo
en Inglés
| IMSEAR
| ID: sea-37242
ABSTRACT
The streptavidin-biotin-peroxidase complex (SABC) technique was compared to conventional indirect immunofluorescence (IIF) for the detection of anti-nuclear antibody (ANA) on HEp-2 cell substrate. SABC showed higher specificity and predictive value and gave more reproducible titres and clearer staining patterns than IIF in sera from a series of rheumatic disease patients. Sera from 80 patients with various types of rheumatic diseases and 20 without rheumatic disease were further tested using the SABC method. All systemic lupus erythematosus (SLE) sera were positive. The overall sensitivity was 95%, specificity 90% and predictive value 97% for rheumatic disease. The rim pattern was associated with SLE and mixed connective tissue disease. The nucleolar/homogeneous pattern was associated with scleroderma and SLE in remission. ANA titre and staining pattern have limited value in the clinical assessment of rheumatic disease; however, ANA has very high sensitivity for SLE and remains an excellent screening test.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Pronóstico
/
Humanos
/
Células Tumorales Cultivadas
/
Anticuerpos Antinucleares
/
Enfermedades Reumáticas
/
Valor Predictivo de las Pruebas
/
Técnica del Anticuerpo Fluorescente
/
Técnicas para Inmunoenzimas
/
Sensibilidad y Especificidad
/
Lupus Eritematoso Sistémico
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Idioma:
Inglés
Revista:
Asian Pac J Allergy Immunol
Año:
1992
Tipo del documento:
Artículo
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