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Development of an enzyme-linked immunosorbent assay for detection of IgE antibodies specific to Dermatophagoides pteronyssinus.
Asian Pac J Allergy Immunol ; 1988 Dec; 6(2): 103-6
Artículo en Inglés | IMSEAR | ID: sea-37248
ABSTRACT
Allergy to Dermatophagoides pteronyssinus was determined in 61 rhinitis patients using prick test (PT), enzyme-immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA). A total of 43 patients tested positive with PT. Forty six patients were positive when tested with EIA and ELISA. With PT as standard test, EIA was found to have 83.7% sensitivity and 44.4% specificity; ELISA had 81.4% sensitivity and 38.9% specificity. There was a linear relationship between absorbance values obtained by EIA and ELISA. The performance time was 8 hours, 24 hours and 30 minutes for ELISA, EIA and PT respectively. The cost per test for ELISA, EIA and PT was US$ 0.20, US$ 5.20 and US$ 0.14 respectively. It was concluded that ELISA was more cost-effective than EIA should be used to supplement PT for a more complete diagnosis of allergy.
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Humanos / Inmunoglobulina E / Ensayo de Inmunoadsorción Enzimática / Pruebas Cutáneas / Técnicas para Inmunoenzimas / Sensibilidad y Especificidad / Análisis Costo-Beneficio / Hipersensibilidad / Animales / Malasia Tipo de estudio: Estudio diagnóstico País/Región como asunto: Asia Idioma: Inglés Revista: Asian Pac J Allergy Immunol Año: 1988 Tipo del documento: Artículo

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Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Humanos / Inmunoglobulina E / Ensayo de Inmunoadsorción Enzimática / Pruebas Cutáneas / Técnicas para Inmunoenzimas / Sensibilidad y Especificidad / Análisis Costo-Beneficio / Hipersensibilidad / Animales / Malasia Tipo de estudio: Estudio diagnóstico País/Región como asunto: Asia Idioma: Inglés Revista: Asian Pac J Allergy Immunol Año: 1988 Tipo del documento: Artículo