Gene amplification using DNA from human spot urine samples.
Artículo
en Inglés
| IMSEAR
| ID: sea-37723
ABSTRACT
The purpose of this study was to test the amplification of DNA from human urinary sediment for molecular epidemiological studies. Twenty-six urine samples were obtained from healthy volunteers. Polymerase chain reactions (PCR) for methylenetetrahydrofolate reductase (MTHFR), beta-globin, and N-acetyltransferase 2 (NAT2) was conducted using genomic DNA isolated from the urine. The MTHFR and beta-globin genes were amplified successfully from all the urine DNA samples while the NAT2 gene was amplified in 88.5% of cases. The median yield of DNA was 0.28 microg from the 10 ml urine samples, sufficient amounts of DNA being contained in urinary sediments for amplification of all three genes. This result indicates that urine can be used as a DNA source for PCR-based molecular epidemiological studies.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Arilamina N-Acetiltransferasa
/
Valores de Referencia
/
Anciano de 80 o más Años
/
Anciano
/
Femenino
/
Humanos
/
Masculino
/
ADN
/
Globinas
/
Niño
Límite:
Aged80
Idioma:
Inglés
Año:
2006
Tipo del documento:
Artículo
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