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Development of enzyme-linked immunosorbent assay for hepatitis B e antigen.
Artículo en Inglés | IMSEAR | ID: sea-38979
ABSTRACT
Hepatitis B e antigen (HBeAg) in sera indicates infectivity and when found during pregnancy, indicates a need for vaccination against hepatitis B virus. A sensitive test for HBeAg is needed in all hospitals but this test is expensive. Local development of enzyme-linked immunosorbent assay (ELISA) for HBeAg and its antibody (anti-HBe) was considered necessary and it was successfully conducted. The developed test was compared with ELISA test for HBeAg and anti-HBe manufactured by Organon Teknika (205 routine specimens and 103 sera positive for HBsAg) and Roche Diagnostic (160 routine specimens). The locally made and imported kits showed overall agreement of 97.5 to 98.1 per cent and the locally made test was always slightly more sensitive. The local test was also rapid, reproducible, and specific. The development lead to self reliance on ELISA test for HBeAg and anti-HBe.
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Tailandia / Femenino / Humanos / Embarazo / Ensayo de Inmunoadsorción Enzimática / Reacciones Falso Positivas / Hepatitis B / Anticuerpos contra la Hepatitis B / Antígenos e de la Hepatitis B País/Región como asunto: Asia Idioma: Inglés Año: 1990 Tipo del documento: Artículo

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Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Tailandia / Femenino / Humanos / Embarazo / Ensayo de Inmunoadsorción Enzimática / Reacciones Falso Positivas / Hepatitis B / Anticuerpos contra la Hepatitis B / Antígenos e de la Hepatitis B País/Región como asunto: Asia Idioma: Inglés Año: 1990 Tipo del documento: Artículo