Hemoglobin E detection using PCR with confronting two-pair primers.
Artículo
en Inglés
| IMSEAR
| ID: sea-39001
ABSTRACT
OBJECTIVES:
To develop and apply the polymerase chain reaction with confronting two-pair primers (PCR-CTPP) for detection and identification of hemoglobin E (Hb E). MATERIAL ANDMETHOD:
Fifty unrelated northern Thais were included in the present study. DNA was extracted from peripheral blood mononuclear cells and targeted to amplify by PCR-CTPP. The amplified product was analyzed and compared with the reference hemoglobin electrophoresis and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis.RESULTS:
The results validated a completely concordant among these three methods consisting of 74%, 24%, and 2% identified as normal, heterozygous, and homozygous Hb E type, respectively.CONCLUSION:
Successful Hb E genotyping by PCR-CTPP was introduced. It allows for confirming and simultaneously detection with other thalassemia mutations.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Tailandia
/
Polimorfismo de Longitud del Fragmento de Restricción
/
Humanos
/
ADN
/
Hemoglobina E
/
Amplificación de Genes
/
Reacción en Cadena de la Polimerasa
/
Talasemia beta
/
Cartilla de ADN
/
Genotipo
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
País/Región como asunto:
Asia
Idioma:
Inglés
Año:
2008
Tipo del documento:
Artículo
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