Green tea polyphenols inhibit oxidized LDL-induced NF-KB activation in human umbilical vein endothelial cells.
Acta Med Indones
;
2007 Apr-Jun; 39(2): 66-70
Artículo
en Inglés
| IMSEAR
| ID: sea-46967
ABSTRACT
AIM:
To examine the anti-inflammatory effects of green tea polyphenols on oxLDL-mediated TNFalpha expression and NF-KB activation in the human umbilical vein endothelial cells (HUVECs).METHODS:
We postulate that green tea polyphenols regulate TNF-alpha gene expression by modulating NF-KB activation through their inhibition effect on IKB Kinase (IKK) activity and as scavenger of free radicals. Pretreatment of green tea polyphenols reduced oxLDL-induced production of proinflammatory cytokine TNF-alpha and NF-KB activation in dose dependent manner (p < 0.05). Post hoc comparison test with Mann Whitney between various dosage of green tea polyphenols in inhibition of NF-KB activation showed significant result (p < 0.05).RESULTS:
In TNF-alpha expression, there was also declined TNF-alpha productions (p 0.09; 0.2 vs 0.4mg/ml ns). The effect of green tea polyphenols on TNF-alpha expression were determined by Mann-Whitney test. There is significant difference between the first dose (0.1mg/ml) vs 0.2mg/ml polyphenols (p=0.009); between 0.1 vs 0.4 mg/ml polyphenols (p=0.009). There was no difference when the dose was increased from 0.2 mg/ml to 0,4 mg/ml polyphenols (0.141). In this study, green tea polyphenols showed significant effects on the inhibition of TNF-alpha through NF-KB activation pathway in HUVECs with oxidized LDL.CONCLUSION:
Green tea polyphenol can be used to prevent the development of atherosclerosis.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Fenoles
/
Arteriosclerosis
/
Té
/
Venas Umbilicales
/
Flavonoides
/
Humanos
/
Expresión Génica
/
FN-kappa B
/
Factor de Necrosis Tumoral alfa
/
Especies Reactivas de Oxígeno
Idioma:
Inglés
Revista:
Acta Med Indones
Año:
2007
Tipo del documento:
Artículo
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