Production of monoclonal antibodies to a tumor--associated antigen by spontaneous cell fusion.
Indian J Cancer
;
2000 Mar; 37(1): 15-22
Artículo
en Inglés
| IMSEAR
| ID: sea-49778
ABSTRACT
Spontaneous cell fusion induced by the bacterium Haemophilus paragallinarum has been recently reported as an alternative technique to generate hybridomas producing monoclonal antibody (mAb). In order to investigate the advantages of this technique to produce anti-tumor monoclonal antibodies we performed comparative experiments between H. paragallinarum induced spontaneous cell fusion and polyethylene glycol (PEG) mediated fusion. Hybridomas producing monoclonal antibodies to an experimental murine lymphoma antigen, the Dalton's lymphoma associated antigen (DLAA) were generated and their sensitivity and specificity were ascertained. The spontaneous fusion yielded more number of stable and specific hybridomas than PEG mediated fusion. The results suggest the advantage of H. paragalinarum induced cell fusion for the simplified production of specific antitumor monoclonal antibodies.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Inmunoglobulina G
/
Inmunoglobulina M
/
Ensayo de Inmunoadsorción Enzimática
/
Antígenos de Histocompatibilidad Clase I
/
Fusión Celular
/
Haemophilus
/
Sensibilidad y Especificidad
/
Linfoma
/
Animales
/
Ratones
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Indian J Cancer
Año:
2000
Tipo del documento:
Artículo
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