Use of polymerase chain reaction (PCR) for detection of Chlamydia trachomatis infection in cervical swab samples.
Indian J Dermatol Venereol Leprol
;
2001 Sep-Oct; 67(5): 246-50
Artículo
en Inglés
| IMSEAR
| ID: sea-52277
ABSTRACT
A polymerase chain reaction (PCR) based method has been set up for detection of Chlamydia trachomatis (C. trachomatis) infection in single cervical swab samples. A primer pair specific to the major outer membrane protein (MOMP) gene common to all serotypes of C. trachomatis was used. This method was validated for its sensitivity as well as specificity. A minimum Ing of DNA could be used for detection of the infection. Specificity of the method was confirmed by carrying out a sample dilution curve. The cervical swab samples analysed in the present study were in coded form for validation of the PCR with an established commercial ELISA (Chlamydiazyme). Both the sensitivity and specificity of PCR was 100% when the ELISA results of these samples were decoded. Thus, this PCR technique could be used for better diagnosis of C. trachomatis infection in comparison to the commercially available ELISA technique.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Indian J Dermatol Venereol Leprol
Año:
2001
Tipo del documento:
Artículo
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