Significance of the hepatitis C virus (HCV) core antigen as an alternative plasma marker of active HCV infection.
Indian J Med Microbiol
;
2007 Jan; 25(1): 37-42
Artículo
en Inglés
| IMSEAR
| ID: sea-53786
ABSTRACT
PURPOSE:
To evaluate the role of core antigen (Ortho trak-C assay) as a marker of active HCV infection in comparison to HCV RNA as detected by reverse transcription polymerase chain reaction (RT-PCR).METHODS:
This evaluation was carried out during January 2000 to December 2003 in HCV infected individuals who were treatment naomicronve or were on anti-viral therapy. Additionally, sequential plasma samples from patients on clinical follow-up were included in this study. A total of 167 samples from 61 patients were tested by trak-C and RT-PCR. HCV RNA detection was achieved by a RT-PCR. Trak-C assay results were also compared in a limited proportion of these samples with known HCV viral load and genotype.RESULTS:
Of 167 samples tested, 56.9% were RNA positive and 43.1% were RNA negative while 50.3% were trak-C positive and 49.7% were trak-C negative, yielding a sensitivity of 85.3% and a specificity of 95.8% for the trak-C assay (Kappa co-efficient = 0.8). The concentration of HCVcAg and HCV RNA showed significant correlation (n=38, r=0.334, P =0.04). The trak-C assay detected the most prevalent HCV genotypes in India without significant difference (P =0.335). The difference between mean absorbance values of HCV RNA positive samples compared to HCV RNA negative samples in the trak-C assay was highly significant (P < 0.000). Qualitative results of trak-C assay and RT-PCR were comparable in 93% of follow-up samples.CONCLUSIONS:
Trak-C assay can be recommended for confirmation of HCV infection and follow-up in laboratories with resource-poor facilities.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
ARN Viral
/
Reproducibilidad de los Resultados
/
Sensibilidad y Especificidad
/
Hepatitis C
/
Hepacivirus
/
Antígenos de la Hepatitis C
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
India
Tipo de estudio:
Estudio diagnóstico
/
Investigación cualitativa
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Indian J Med Microbiol
Asunto de la revista:
Microbiology
Año:
2007
Tipo del documento:
Artículo
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